NucleoBeads
Profiling DNA-binding proteins and transcription factors
- DNA binding proteins, especially transcription factors, act as major oncodrivers and represent an important therapeutic target class
- NucleoBeads™ is a quantitative chemical proteomics technique that enables comprehensive quantification of proteins binding selectively to specific nucleotide sequences (DNA or RNA)
- Using a proprietary mix of double-strand DNA oligonucleotide probes, we reproducibly quantify >300 transcription factors and transcription factor complexes, e.g., P53, NFkB, and MYC
- NucleoBeads™ bound proteins can be displaced by compounds or free nucleotide sequences in a competition assay to uncover their molecular (off-)targets and to determine affinity and selectivity values
- The assay can be used to also identify compounds modulating transcription factor complex composition as well as compounds inducing activity changes of DNA-binding proteins
Due to its high-throughput capabilities, this technology is well suited to screen large compound libraries.
The assay can be readily adapted to new transcription factor classes.
Further reading
Ruprecht B., et al., Chemoproteomic profiling to identify activity changes and functional inhibitors of DNA-binding proteins. Cell Chem Biol. 2022;29(11):1639-1648.e4 [Link]
NucleoBeads example results
NucleoBeads enrich hundreds of transcription factors and DNA-binding proteins. They can be used to identify functional inhibitors of DNA-binding proteins and modulators of transcription factor activities.